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Diaphragm Definition The diaphragm is a sheet of muscle that lies at the bottom of the chest cavity. It separates the chest cavity from the abdominal cavity, and is responsible for breathing. The diaphragm contracts and relaxes to move air into and out of the lungs. It also plays a role in other functions such as digestion, coughing, and urination. It is an important part of the body's respiratory system.